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A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
15:04

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity

Published on: May 5, 2009

Human cell-based micro electrode array platform for studying neurotoxicity.

Laura Ylä-Outinen1, Juha Heikkilä, Heli Skottman

  • 1Regea - Institute for Regenerative Medicine, University of Tampere and Tampere University Hospital Tampere, Finland.

Frontiers in Neuroengineering
|October 19, 2010
PubMed
Summary

This study introduces a human cell-based microelectrode array (MEA) platform for real-time neurotoxicology screening. The MEA method detected methyl mercury chloride

Keywords:
human embryonic stem cellmethyl mercury chloridemicroelectrode arrayneuronal networkneurotoxicology

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Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line
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Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line

Published on: August 9, 2014

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Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line
08:23

Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line

Published on: August 9, 2014

Area of Science:

  • Neuroscience
  • Toxicology
  • Stem Cell Biology

Background:

  • Current neurotoxicology relies heavily on animal models and in vitro molecular endpoints.
  • There is a critical need for human cell-based platforms to assess functional neuronal network responses to neurotoxicants in real-time.
  • Microelectrode array (MEA) technology allows for long-term monitoring of in vitro neuronal network activity.

Purpose of the Study:

  • To investigate the neurotoxicity of methyl mercury chloride (MeHgCl) using a human embryonic stem cell (hESC)-derived neuronal network on an MEA platform.
  • To evaluate the sensitivity of MEA for detecting functional changes in neuronal networks exposed to neurotoxicants.
  • To compare MEA findings with traditional molecular assays for neurotoxicity assessment.

Main Methods:

  • Human embryonic stem cell (hESC)-derived neuronal networks were cultured on microelectrode arrays (MEAs).
  • Networks exhibiting spontaneous electrical activity were exposed to methyl mercury chloride (MeHgCl) at concentrations ranging from 0.5–500 nM for 72 hours.
  • MEA recordings were performed acutely and at 24, 48, and 72 hours post-exposure, followed by molecular analyses (proliferation, survival, gene/protein expression).

Main Results:

  • A concentration of 500 nM MeHgCl significantly decreased electrical signaling in hESC-derived neuronal networks.
  • The neurotoxic effects of MeHgCl on network function were observed in a delayed manner.
  • Traditional molecular methods (qRT-PCR, immunostaining, proliferation assays) did not detect the neurotoxic effects observed with MEA.

Conclusions:

  • The human cell-based MEA platform is a sensitive and effective online tool for real-time neurotoxicological screening.
  • MEA technology can detect functional neurotoxicity that may be missed by conventional molecular assays.
  • This platform offers a promising alternative for assessing the impact of neurotoxicants on human neuronal networks.