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Updated: Jan 22, 2026

Use of In Vivo Assembly for High-efficiency Plasmid Construction
Published on: February 7, 2025
CABYR RNAi plasmid construction and NF-κB signal transduction pathway
Lin-Xiang Shi1, Yao-Ming He, Lin Fang
1Department of General Surgery, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, China. ajijinwei@126.com
Aim:
To construct the CABYR RNAi plasmid and study its relation with the nuclear factor (NF)-κB signal transduction pathway.
Methods:
Human CABYR mRNA sequence was obtained from GenBank. The structure of cDNA sequence for the short hairpin RNA was BbsI + sense + loop + antisense + transcription terminator + KpnI + BamHI. A CABYR silencing plasmid was constructed and transfected into the human embryo cell line 293T. Quantitative real-time polymerase chain reaction was used to analyze CABYR and NF-κB gene expression.
Results:
The CABYR and NF-κB expressions were detected in 293T cells. The oligonucleotide (5'-GCTCAGATGTTAGGTAAAG-3') efficiently silenced the expression of CABYR. The expression of NF-κB was not significantly affected by silencing CABYR (P = 0.743).
Conclusion:
CABYR can be found in the human embryo cell line 293T. Cabyrmid 2 can efficiently silence its target, CABYR, indicating that CABYR is not related with the NF-κB signal transduction pathway.
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