Related Experiment Video
Updated: Jun 8, 2026
07:36
Analyzing Tumor and Tissue Distribution of Target Antigen Specific Therapeutic Antibody
Published on: May 16, 2020
[Targeted therapy by monoclonal antibodies]
1Department of Rheumatology and Allergy, Research Hospital, Institute of Medical Science, University of Tokyo.
Nihon Rinsho. Japanese Journal of Clinical Medicine
|October 20, 2010
Summary
Human monoclonal antibodies are crucial for treating various diseases. This review covers current techniques for producing effective therapeutic monoclonal antibodies, addressing challenges in their selection for in vivo use.
Area of Science:
- Immunology and Biotechnology
- Therapeutic Antibody Development
Context:
- Monoclonal antibodies are vital for cancer, infectious, and autoimmune disease immunotherapies.
- The hybridoma technique, while effective for murine antibodies, has limitations for human therapeutic use.
- Advancements in biotechnology have improved human monoclonal antibody production efficiency.
Purpose:
- To provide an overview of current techniques for therapeutic monoclonal antibody production.
- To highlight challenges in selecting effective in vivo therapeutic antibodies.
- To discuss strategies for enhancing the efficacy of human monoclonal antibodies.
Summary:
- Discusses the indispensable role of human monoclonal antibodies in modern medicine.
- Reviews the evolution from murine antibody production (hybridoma technique) to advanced human antibody manufacturing.
- Addresses the critical need for effective in vivo selection strategies for therapeutic antibodies.
Impact:
- Facilitates understanding of current therapeutic monoclonal antibody technologies.
- Aims to guide the development of more effective antibody-based treatments.
- Supports the advancement of immunotherapy for diverse medical conditions.
Related Concept Videos
02:57Targeted Cancer Therapies
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...
There are several types of targeted therapies against specific...
02:57Targeted Cancer Therapies
The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...
There are several types of targeted therapies against specific...
01:27Tumor Immunotherapy
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
01:31Hybridoma Technology
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
01:24Modified-Release Drug Delivery Systems: Site-Targeted
Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.
02:50Combination Therapies and Personalized Medicine
Combining two or more treatment methods increases the life span of cancer patients while reducing damage to vital organs or tissue from the overuse of a single treatment. Combination therapy also targets different cancer-inducing pathways, thus reducing the chances of developing resistance to treatment.
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...
The combination of the drug acetazolamide and sulforaphane is a good example of combination therapy to treat cancer. The cells in the interior of a large tumor often die due to the hypoxic and...