Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Variables influencing Streptococcus mutans testing.

S J Weinberger1, G Z Wright

  • 1Faculty of Dentistry, University of Western Ontario, London, Canada.

Pediatric Dentistry
|September 1, 1990
PubMed
Summary

The tongue depressor method is reliable for sampling saliva to count Streptococcus mutans (S. mutans). However, saliva S. mutans counts vary significantly within individuals throughout the day.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The safety and efficacy of treatment with air abrasion technology.

International journal of paediatric dentistry·1999
Same author

The management of premolar supernumeraries in three orthodontic cases.

The Journal of clinical pediatric dentistry·1998
Same author

Microleakage of sealants after conventional, bur, and air-abrasion preparation of pits and fissures.

Pediatric dentistry·1998
Same author

The effect of internal bevel on marginal leakage at the approximal surface of Class 2 composite restorations.

Operative dentistry·1998
Same author

Bond strengths of two ceramic brackets using argon laser, light, and chemically cured resin systems.

The Angle orthodontist·1997
Same author

Management of lower incisor crowding in the early mixed dentition.

ASDC journal of dentistry for children·1996

Area of Science:

  • Microbiology
  • Pediatric Dentistry
  • Oral Health Research

Background:

  • Estimating Streptococcus mutans (S. mutans) levels in saliva is crucial for assessing caries risk.
  • Saliva sampling methods can be influenced by various factors, potentially affecting S. mutans quantification.

Purpose of the Study:

  • To evaluate the reliability of tongue depressors as a tool for collecting saliva samples for S. mutans analysis.
  • To determine if the time of day influences S. mutans counts in pediatric saliva samples.

Main Methods:

  • Unstimulated saliva samples were collected from 27 children (24-66 months) using tongue blades, four times daily.
  • Paired saliva samples from tongue blades were cultured on selective agar and incubated anaerobically.
  • Statistical analysis, including analysis of variance, was used to assess S. mutans counts.

Main Results:

  • No significant difference in S. mutans counts was observed between paired sides of the tongue blades, indicating sampling technique suitability.
  • Significant variations in S. mutans counts were found within individual subjects across the four daily sampling times (P < .01).
  • Significant differences were also detected between the daily average S. mutans counts of different subjects (P < .05).

Conclusions:

  • Tongue depressor saliva sampling is a reliable method for S. mutans enumeration.
  • Diurnal variations in S. mutans levels are significant in young children, necessitating consideration of sampling time.
  • Further research may explore factors contributing to diurnal S. mutans fluctuations.

Related Experiment Videos