Related Experiment Video

Updated: Jun 7, 2026

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death
09:18

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Published on: December 27, 2016

Altered expression of signaling genes in Jurkat cells upon FTY720 induced apoptosis

Fang Wang1, Wenfeng Tan, Dunming Guo

  • 1The Lab of Cardiology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;

International Journal of Molecular Sciences
|October 20, 2010
PubMed

Insights

FTY720, an immunosuppressant, induces Jurkat cell apoptosis via mitochondrial signaling pathways. This study identifies key genes like MOAP-1 and VEGF involved in FTY720

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • FTY720 is a novel immunosuppressant affecting T lymphocytes.
  • FTY720's mechanism for inducing lymphocyte apoptosis is not fully understood.
  • Investigating apoptotic signaling pathways is crucial for understanding FTY720's action.

Purpose of the Study:

  • To elucidate the cell signaling mechanisms of FTY720-induced apoptosis in Jurkat cells.
  • To identify specific genes and pathways modulated by FTY720.
  • To provide a mechanistic insight into FTY720's immunosuppressive effects.

Main Methods:

  • Jurkat cells were treated with FTY720.
  • Apoptosis was assessed using cell viability, Hoechst staining, Annexin V binding, and DNA fragmentation assays.
  • cDNA microarray analysis and reverse transcription polymerase chain reaction (RT-PCR) were employed to examine gene expression.

Main Results:

  • FTY720 induced Jurkat cell apoptosis in a dose- and time-dependent manner.
  • Microarray analysis revealed significant up-regulation of 54 genes and down-regulation of 10 genes related to apoptosis.
  • Key genes such as MOAP-1, VEGF, TRAF 6, CASP 2, E2F 1, and CASP 5 showed increased expression.

Conclusions:

  • Mitochondria-related signaling pathways are central to FTY720-induced apoptosis in Jurkat cells.
  • The study provides novel insights into the molecular mechanisms of FTY720.
  • A potential signaling pathway diagram for FTY720-mediated apoptosis was proposed.
Keywords:
FTY720apoptosiscDNA microarrayjurkat cellsignal transduction

More Related Videos

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants07:38

Intracellular Phosphoflow Cytometry of Acute Myeloid Leukemia Patient-Derived Xenotransplants

Published on: June 6, 2025

Rapid In Vitro Cytotoxicity Evaluation of Jurkat Expressing Chimeric Antigen Receptor using Fluorescent Imaging09:20

Rapid In Vitro Cytotoxicity Evaluation of Jurkat Expressing Chimeric Antigen Receptor using Fluorescent Imaging

Published on: October 27, 2023

Related Concept Videos

The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Apoptosis01:30

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size reduction of the tissue.
Cellular Injury V: Apoptosis and Autophagy01:22

Cellular Injury V: Apoptosis and Autophagy

Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...