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siRNA - Small Interfering RNAs

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Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
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RNAi screen indicates widespread biological function for human natural antisense transcripts.

Mohammad Ali Faghihi1, Jannet Kocerha, Farzaneh Modarresi

  • 1Department of Neuroscience, The Scripps Research Institute, Jupiter, Florida, United States of America.

Plos One
|October 20, 2010
PubMed
Summary
This summary is machine-generated.

Natural antisense transcripts are regulatory RNA molecules. A large RNA interference screen revealed their significant role in regulating cell viability and signaling pathways, impacting fundamental cellular events.

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Area of Science:

  • Molecular Biology
  • Genomics
  • RNA Biology

Background:

  • Natural antisense transcripts (NATs) are regulatory RNA molecules with complementary sequences to other RNA transcripts.
  • NATs are prevalent in the mammalian genome, but their biological functions remain largely undefined.

Purpose of the Study:

  • To investigate the biological significance and regulatory roles of natural antisense transcripts.
  • To evaluate the potential of large-scale RNA interference screens for studying NAT function.

Main Methods:

  • A loss-of-function RNA interference (RNAi) screen was conducted.
  • The screen targeted 797 evolutionarily conserved antisense transcripts using small interfering RNA (siRNA).

Main Results:

  • Evidence for a regulatory role was found for several natural antisense transcripts.
  • NATs targeting CCPG1 and RAPGEF3 were shown to disrupt signaling pathways and affect cell viability.
  • These effects occurred independently or in parallel with their corresponding sense transcripts.

Conclusions:

  • Large-scale siRNA screening is a viable method for assessing the functional modulation of natural antisense transcripts.
  • NATs play a significant role in regulating fundamental cellular processes, including cell viability and signaling pathways.