Monolysocardiolipin: improved preparation with high yield
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Junhwan Kim1, Charles L Hoppel
1Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106, USA.
Journal of Lipid Research
|October 21, 2010
Abstract:
A simple, high-yielding preparation of monolysocardiolipin (MLCL) by phospholipase A2 hydrolysis of cardiolipin (CL) in methanol on a semi-preparative scale is described. In methanol, phospholipase A2 preferentially hydrolyzes CL to MLCL. This selectivity results in ∼80% yield of MLCL. The synthesized MLCL and dilysocardiolipin were characterized by NMR and ESI-MS/MS. Only the sn-2 position of CL was hydrolyzed by phospholipase A2 in methanol.
