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Updated: Jun 7, 2026
11:55
In Vitro Reconstitution of Light-harvesting Complexes of Plants and Green Algae
Published on: October 10, 2014
Preparation of native and recombinant light-harvesting chlorophyll-a/b complex
Wolfgang Rühle1, Harald Paulsen
1Institut für Allgemeine Botanik, Johannes-Gutenberg-Universität, Mainz, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|October 21, 2010
Abstract:
Procedures to isolate native light-harvesting chlorophyll-a/b complex (LHCIIb) and to reconstitute recombinant LHCIIb are described. Separation of trimeric from monomeric forms and free pigment by sucrose density-gradient ultracentrifugation can be applied to both native and reconstituted complexes. The preparations are characterized by their pigment composition, protein pattern, and spectral properties.
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