Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Canine thyroid fucokinase.

W L Richards, G S Serif

    Biochimica Et Biophysica Acta
    |October 13, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a radiometric assay to detect and purify canine thyroid fucokinase (ATP:6-deoxy-L-galactose 1-phosphotransferase, EC 2.7.1.52). The enzyme demonstrates high specificity for L-fucose and ATP, requiring divalent cations for activity.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Four words that could ruin your disability protection.

    Medical economics·1994
    Same author

    Hazard evaluation of chemicals that cause accumulation of alpha 2u-globulin, hyaline droplet nephropathy, and tubule neoplasia in the kidneys of male rats.

    Environmental health perspectives·1993
    Same author

    Long-term course of surgically induced astigmatism.

    Journal of cataract and refractive surgery·1988
    Same author

    Differential effects of transforming growth factor-beta on proliferation of normal and malignant rat liver epithelial cells in culture.

    Cancer research·1986
    Same author

    The effects of nonfixated lower lens haptics of Binkhorst lenses on corneal endothelial cell density.

    Ophthalmic surgery·1986
    Same author

    Differences between men and women as related to intraocular lens implantation.

    Ophthalmic surgery·1986

    Area of Science:

    • Biochemistry
    • Enzymology

    Background:

    • Fucokinase (ATP:6-deoxy-L-galactose 1-phosphotransferase, EC 2.7.1.52) plays a role in carbohydrate metabolism.
    • Understanding fucokinase activity is crucial for metabolic pathway research.

    Purpose of the Study:

    • To develop a radiometric assay for fucokinase.
    • To detect and purify fucokinase from canine thyroid tissue.
    • To characterize the kinetic and physical properties of the purified enzyme.

    Main Methods:

    • Development of a radiometric assay using L-[14C]fucose.
    • Purification of fucokinase from canine thyroid extracts.
    • Enzyme characterization including pH optimum, substrate specificity, and molecular weight determination.

    Main Results:

    Related Experiment Videos

    • A sensitive radiometric assay was established.
    • Canine thyroid tissue contains fucokinase, purified 2754-fold.
    • The enzyme exhibits optimal activity at pH 6.5 and 8.25, is specific for L-fucose and ATP, and requires divalent cations (Mg2+ > Mn2+).
    • The molecular weight was determined to be 494,000 ± 12,400 Da.

    Conclusions:

    • The developed assay is effective for fucokinase detection and purification.
    • The characterized canine thyroid fucokinase possesses specific kinetic properties.
    • This study provides foundational data on fucokinase from a mammalian thyroid source.