Related Experiment Video
Updated: Jun 7, 2026
09:16
Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants
Published on: February 21, 2015
Array-based technology and recommendations for utilization in medical genetics practice for detection of chromosomal
Melanie Manning1, Louanne Hudgins,
1Department of Pathology, Stanford University School of Medicine, Stanford, California 94305, USA. mmanning@stanford.edu
Summary
Microarray technologies significantly improve genetic analysis for developmental delay and congenital anomalies. These advanced methods offer higher precision in detecting chromosomal imbalances compared to older techniques.
Area of Science:
- Genetics
- Molecular Biology
- Clinical Diagnostics
Background:
- Laboratory evaluation for developmental delay/intellectual disability, congenital anomalies, and dysmorphic features has evolved.
- Traditional G-banded chromosome studies have limitations in detecting small genetic variations.
Purpose of the Study:
- To review the utility and limitations of microarray technologies in clinical genetic evaluation.
- To provide recommendations for the current and future application of these techniques.
Main Methods:
- Examination of patient genomes for gains or losses of genetic material using microarray technologies.
- Comparison of microarray resolution with standard G-banded chromosome studies.
Main Results:
- Microarray technologies offer increased resolution, precision, accuracy, and technical sensitivity.
- These techniques can identify chromosomal imbalances missed by conventional cytogenetic analysis.
Conclusions:
- Microarray-based platforms are increasingly utilized in clinical practice.
- Strategic utilization and understanding of limitations are crucial for optimal application.
Related Concept Videos
01:17Karyotyping
Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...
01:17Karyotyping
Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...
02:34DNA Microarrays
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...