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Updated: Jun 7, 2026
08:26
Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
Published on: November 23, 2021
Two-photon fluorescent probes for metal ions.
1Division of Energy Systems Research, Ajou University, Suwon, 443-749 Korea. kimhm@ajou.ac.kr
Chemistry, an Asian Journal
|October 22, 2010
Summary
This review highlights two-photon probes for detecting intracellular metal ions in live cells and tissues. It offers design guidelines for developing advanced probes for in vivo imaging applications.
Area of Science:
- Biomedical Imaging
- Chemical Biology
- Molecular Probes
Background:
- Two-photon microscopy (TPM) enables long-term imaging of intact tissues, crucial for biological and medical research.
- Developing specialized two-photon probes enhances TPM's versatility for specific biological questions.
- Detecting intracellular free metal ions is vital for understanding cellular processes.
Purpose of the Study:
- To review recent advancements in two-photon probes for detecting intracellular free metal ions.
- To provide a guideline for designing novel two-photon probes for in vivo imaging.
- To showcase model studies and selected examples of metal ion-detecting probes.
Main Methods:
- Literature review of recent research on two-photon probes.
- Analysis of model studies demonstrating probe performance.
- Selection and summary of exemplary two-photon probes for metal ion detection.
Main Results:
- Recent studies have yielded effective two-photon probes for various intracellular metal ions.
- These probes demonstrate successful detection in live cells and tissues.
- Model studies provide insights into probe design principles and limitations.
Conclusions:
- Two-photon probes are essential for advancing in vivo imaging of metal ions.
- The summarized findings offer a roadmap for designing next-generation probes.
- Further development of these probes will enhance their utility in biological and medical research.
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