Related Experiment Video
Updated: Jun 7, 2026

Taste Exam: A Brief and Validated Test
Published on: August 17, 2018
The human bitter taste receptor, hTAS2R16, discriminates slight differences in the configuration of disaccharides
Takanobu Sakurai1, Takumi Misaka, Yohei Ueno
1General Research Institute of Food Science and Technology, Nissin Foods Holdings Co. Ltd., Shiga, Japan.
Abstract:
Sweetness and bitterness are key determinants of food acceptance and rejection, respectively. Sugars, such as sucrose and fructose, are generally recognized as sweet. However, not all sugars are sweet, and even anomers may have quite different tastes. For example, gentiobiose is bitter, whereas its anomer, isomaltose, is sweet. Despite this unique sensory character, the molecular basis of the bitterness of gentiobiose remains to be clarified. In this study, we used calcium imaging analysis of human embryonic kidney 293T cells that heterologously expressed human taste receptors to demonstrate that gentiobiose activated hTAS2R16, a bitter taste receptor, but not hT1R2/hT1R3, a sweet taste receptor. In contrast, isomaltose activated hT1R2/hT1R3. As a result, these anomers elicit different taste sensations. Mutational analysis of hTAS2R16 also indicated that gentiobiose and β-D-glucopyranosides, such as salicin share a common binding site of hTAS2R16.
Related Concept Videos
The Physiology of Taste
Gustation
Taste Buds and Receptors
The Tongue and Taste Buds
G-Protein Gated Ion Channels
Sensory organs,...
Tactile and Chemical Senses

