Related Experiment Video
Updated: Jun 7, 2026

Optimized Preparation of Whole Murine Tumor-Bearing Lung Tissue for Flow Cytometry and Single-Cell RNA-Sequencing
Published on: May 22, 2026
[Molecular biology of lung cancer series]
S Hiret1, H Senellart, J Bennouna
1Centre régional de lutte contre le cancer René-Gauducheau, boulevard Jacques-Monod, 44805 Saint-Herblain, France.
Introduction:
MAGE-A3 (Melanoma Associated Antigen-A3) is expressed in cancer cells but not in normal tissues except male germ line cells which are devoid of Major Histocompatibility Complex molecules and therefore do not present MAGE-A3 antigens.
Background:
MAGE-A3 is expressed in 30 to 60% of non-small cell lung cancers but its function is unknown. Its recognition by cytotoxic T lymphocytes implies its presentation on the cell surface by HLA type A1 molecules that are absent from germ cells.
Viewpoints:
MAGE-A3 represents a good target for active anticancer immunotherapy. Some trials, which used MAGE-A3 and an adjuvant showed a strong antigen-specific T-cell response with, perhaps, an improved survival.
Conclusion:
This needs to be confirmed as an adjuvent therapy by current phase III randomized controlled trials.
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