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Related Concept Videos

pH Homeostasis01:31

pH Homeostasis

Acid-base homeostasis is essential for maintaining normal physiological activities in humans. The pH of various body fluids is strictly regulated because it is critical for the optimal activity of enzymes involved in metabolic reactions. Enzymes are basically proteins, so, any significant change in pH can affect their structure and activity. In humans, pH is regulated using three primary mechanisms— chemical buffer systems, respiratory regulation, and renal regulation.
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Respiratory Regulation of Acid-Base Balance01:18

Respiratory Regulation of Acid-Base Balance

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Chemical Factors Affecting Respiration Centers01:31

Chemical Factors Affecting Respiration Centers

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GPCRs Regulate Adenylyl Cylase Activity01:09

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Two...
Enteric Nervous System: Regulation of GI Motor Activity01:11

Enteric Nervous System: Regulation of GI Motor Activity

The Enteric Nervous System (ENS) plays a pivotal role in regulating gastrointestinal or GI motor activity. This complex network of nerves, deeply embedded within the gut wall, responds to changes in the gut environment and receives input from both the autonomic nervous system and the central nervous system. By doing so, the ENS operates various programs tailored to the body's nutritional status and needs.
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Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

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Related Experiment Video

Updated: Jun 7, 2026

A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines
06:59

A Fluorescence-Based Assay of Membrane Potential for High-Throughput Functional Study of Two Endogenous Ion Channels in Two Epithelial Cell Lines

Published on: June 22, 2022

Pendrin modulates ENaC function by changing luminal HCO3-.

Vladimir Pech1, Truyen D Pham, Seongun Hong

  • 1Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA.

Journal of the American Society of Nephrology : JASN
|October 23, 2010
PubMed
Summary
This summary is machine-generated.

Pendrin influences epithelial sodium channel (ENaC) activity by affecting bicarbonate levels. Restoring bicarbonate levels independently of pendrin rescues ENaC function in pendrin-null mice.

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Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)

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Area of Science:

  • Nephrology
  • Molecular Biology
  • Physiology

Background:

  • The epithelial sodium channel (ENaC) and the chloride/bicarbonate exchanger (pendrin) are key regulators of NaCl absorption in the kidney.
  • Pendrin and ENaC are expressed in different cell types, but ENaC function is reduced in pendrin-null mice.

Purpose of the Study:

  • To investigate whether increasing distal bicarbonate delivery, independent of pendrin, can restore ENaC function in pendrin-null mice.
  • To determine the direct effect of bicarbonate concentration on ENaC abundance and activity in kidney cells.

Main Methods:

  • Administered aldosterone and sodium bicarbonate, with or without acetazolamide, to mice to manipulate bicarbonate levels.
  • Assessed urinary pH, bicarbonate concentration, and ENaC abundance and function.
  • Utilized cultured mouse principal cells (mpkCCD) and Xenopus kidney cells to study the direct impact of bicarbonate on ENaC.

Main Results:

  • Pendrin-null mice treated with aldosterone and sodium bicarbonate showed reduced urinary pH, bicarbonate, and impaired ENaC function compared to wild-type mice.
  • Addition of acetazolamide normalized acid-base balance and ENaC function in pendrin-null mice.
  • Increased apical or basolateral bicarbonate concentration enhanced ENaC abundance and activity in cultured kidney cells, with greater sensitivity to basolateral changes.

Conclusions:

  • Pendrin modulates ENaC abundance and function, partly through regulating luminal bicarbonate concentration and pH.
  • Bicarbonate levels directly influence ENaC activity and expression in renal principal cells.