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Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies
06:56

Isolation and Characterization of Neutrophil-derived Microparticles for Functional Studies

Published on: March 2, 2018

Microparticles initiate decompression-induced neutrophil activation and subsequent vascular injuries

Stephen R Thom1, Ming Yang, Veena M Bhopale

  • 1Institute for Environmental Medicine, Univ. of Pennsylvania, 1 John Morgan Bldg., 3620 Hamilton Walk, Philadelphia, PA 19104-6068, USA. sthom@mail.med.upenn.edu

Journal of Applied Physiology (Bethesda, Md. : 1985)
|October 23, 2010
PubMed

Insights

Decompression stress increases circulating microparticles (MPs), which activate neutrophils and cause vascular damage. Reducing these MPs mitigates decompression-induced injury, highlighting MPs as key players in this process.

Area of Science:

  • Biomedical research
  • Cellular biology
  • Physiology

Background:

  • Decompression stress is known to cause physiological changes.
  • Microparticles (MPs) are circulating cell fragments implicated in various pathological processes.

Purpose of the Study:

  • To investigate the role of annexin V-coated microparticles (MPs) in decompression stress-induced vascular injury.
  • To explore potential therapeutic strategies targeting MPs.

Main Methods:

  • Assessed circulating MP levels in mice under varying decompression stresses.
  • Investigated MP-neutrophil interactions and the effect of polyethylene glycol (PEG) and anti-annexin V antibody on MP levels and vascular injury.

Main Results:

  • Progressive elevation of annexin V-coated MPs from various cell types with increasing decompression.
  • MPs, particularly platelet-derived ones, induced neutrophil degranulation and further MP production.
  • MP reduction via PEG or anti-annexin V antibody decreased neutrophil activation, sequestration, and tissue injury.

Conclusions:

  • Decompression-induced MPs precipitate neutrophil activation and vascular damage.
  • Targeting MPs offers a potential therapeutic approach for decompression-related injuries.

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