Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 7, 2026

SDS-PAGE/Immunoblot Detection of Aβ Multimers in Human Cortical Tissue Homogenates using Antigen-Epitope Retrieval
10:48

SDS-PAGE/Immunoblot Detection of Aβ Multimers in Human Cortical Tissue Homogenates using Antigen-Epitope Retrieval

Published on: April 23, 2010

Detecting aβ*56 oligomers in brain tissues.

Mathew A Sherman1, Sylvain E Lesné

  • 1Department of Neuroscience, Institute for Translational Neuroscience, University of Minnesota, Minneapolis, MN, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 23, 2010
PubMed
Summary

Researchers identified a specific amyloid-beta (Aβ) oligomer, Aβ*56, in the brain. Its levels correlate with cognitive decline, suggesting a key role in Alzheimer's disease pathology beyond plaques.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Longevity Factor Klotho and Resistance to Cognitive Deficits in Individuals with Parkinson's Disease and in an α-Synuclein Mouse Model.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2026
Same author

Retraction notice to "Cognitive effects of cell-derived and synthetically-derived Aβ oligomers" [Neurobiol. Aging 32 (2009) 1784-1794].

Neurobiology of aging·2026
Same author

Standard diet and animal source influence hippocampal spatial reference learning and memory in congenic C57BL/6J mice.

Research square·2024
Same author

Correction: SNCA genetic lowering reveals differential cognitive function of alpha-synuclein dependent on sex.

Acta neuropathologica communications·2024
Same author

SNCA genetic lowering reveals differential cognitive function of alpha-synuclein dependent on sex.

Acta neuropathologica communications·2022
Same author

Comment on "Activation of the Omega-3 Fatty Acid Receptor GPR120 Protects against Focal Cerebral Ischemic Injury by Preventing Inflammation and Apoptosis in Mice".

Journal of immunology (Baltimore, Md. : 1950)·2022

Area of Science:

  • Neuroscience
  • Biochemistry
  • Pathology

Background:

  • Alzheimer's disease (AD) has been historically linked to amyloid plaques and neurofibrillary tangles.
  • However, the correlation between plaque burden and cognitive impairment in humans is weak.
  • This suggests other pathological factors, potentially soluble amyloid-beta (Aβ) species, may be involved.

Purpose of the Study:

  • To investigate the role of soluble amyloid-beta (Aβ) species in cognitive decline associated with aging and Alzheimer's disease.
  • To biochemically isolate and characterize a specific Aβ oligomer implicated in cognitive impairment.

Main Methods:

  • Utilized a multistep fractionation protocol to isolate specific Aβ species from brain tissue.
  • Employed biochemical techniques to identify and characterize oligomeric forms of Aβ.

Main Results:

  • Identified a 56 kDa oligomer of amyloid-beta (Aβ), designated Aβ*56.
  • Demonstrated that the amount of Aβ*56 in brain tissue correlates with cognitive impairment.
  • Successfully isolated this specific oligomeric Aβ species from transgenic mouse models of AD.

Conclusions:

  • Soluble amyloid-beta (Aβ) oligomers, particularly Aβ*56, represent a significant pathological factor in Alzheimer's disease.
  • Aβ*56 levels serve as a potential biomarker for cognitive dysfunction in AD.
  • This finding shifts focus towards soluble Aβ species as key drivers of AD pathogenesis.

More Related Videos

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis
06:17

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

Published on: May 22, 2018

Related Experiment Videos

Last Updated: Jun 7, 2026

SDS-PAGE/Immunoblot Detection of Aβ Multimers in Human Cortical Tissue Homogenates using Antigen-Epitope Retrieval
10:48

SDS-PAGE/Immunoblot Detection of Aβ Multimers in Human Cortical Tissue Homogenates using Antigen-Epitope Retrieval

Published on: April 23, 2010

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis
06:17

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

Published on: May 22, 2018