Jove
Visualize
Contact Us

Related Concept Videos

Imaging Studies for Cardiovascular System V: CT01:28

Imaging Studies for Cardiovascular System V: CT

Cardiac computed tomography (CT) scanning is an advanced cardiac imaging technique that utilizes CT technology, with or without intravenous (IV) contrast, to produce accurate cross-sectional virtual slices of specific areas of the heart, coronary circulation, and major blood vessels such as the aorta, pulmonary veins, and arteries. The computer processes these slices to generate three-dimensional images. Multidetector CT (MDCT) is a rapid form of CT scanning that captures multiple slices...
Imaging Studies for Cardiovascular System VI: Calcium -Scoring CT01:25

Imaging Studies for Cardiovascular System VI: Calcium -Scoring CT

Calcium-Scoring CT ScanA calcium-scoring CT scan, also known as coronary artery calcium (CAC) scan, detects calcium deposits in the coronary arteries. This test assesses the risk of coronary artery disease (CAD), which can lead to cardiovascular events such as angina, heart failure, and sudden cardiac arrest.A calcium-scoring CT scan is generally recommended for individuals at intermediate risk of CAD without symptoms. It includes:Men aged 40-75 and women aged 50-75: Especially those with a...
Imaging Studies III: Computed Tomography01:27

Imaging Studies III: Computed Tomography

DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
Imaging Studies VII: Vascular Imaging01:19

Imaging Studies VII: Vascular Imaging

DefinitionRenal angiography, also known as renal arteriography, is an imaging technique used to obtain a comprehensive view of blood flow and the vascular structure of blood vessels in the kidneys and surrounding areas.PurposeRenal angiography detects blood vessel abnormalities in the kidneys, such as aneurysms, stenosis, thrombosis, vascular tumors, and renal artery stenosis. It evaluates kidney function and guides interventional treatments like angioplasty or stent placement.Pre-Procedure...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineered split in Pfu DNA polymerase fingers domain improves incorporation of nucleotide gamma-phosphate derivative.

Nucleic acids research·2010
Same author

Fine-tuning enzyme activity through saturation mutagenesis.

Methods in molecular biology (Clifton, N.J.)·2010
Same author

Easy two-step method for randomizing and cloning gene fragments.

Methods in molecular biology (Clifton, N.J.)·2010
Same author

Rapid quantification of DNA libraries for next-generation sequencing.

Methods (San Diego, Calif.)·2010
Same author

Escherichia coli DNA polymerase III epsilon subunit increases Moloney murine leukemia virus reverse transcriptase fidelity and accuracy of RT-PCR procedures.

Analytical biochemistry·2006
Same author

Amplification efficiency of thermostable DNA polymerases.

Analytical biochemistry·2003
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 25, 2026

Neuro-rehabilitation Approach for Sudden Sensorineural Hearing Loss
09:44

Neuro-rehabilitation Approach for Sudden Sensorineural Hearing Loss

Published on: January 26, 2016

Long-range PCR with a DNA polymerase fusion.

Holly H Hogrefe1, Michael C Borns

  • 1Stratagene Products Division, Agilent Technologies, Inc, La Jolla, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 23, 2010
PubMed
Summary
This summary is machine-generated.

Proofreading DNA polymerase fusions enable faster, high-fidelity long-range PCR for genomic targets over 20 kb. This modified protocol generates high-molecular-weight amplicons efficiently in a single day.

More Related Videos

Systematic Hearing Performance Evaluation Process for Adolescents with Cochlear Implantation at Early Ages
06:04

Systematic Hearing Performance Evaluation Process for Adolescents with Cochlear Implantation at Early Ages

Published on: March 24, 2023

An Automated System for Sound Localization Testing in Hearing-Impaired Listeners
07:52

An Automated System for Sound Localization Testing in Hearing-Impaired Listeners

Published on: March 13, 2026

Related Experiment Videos

Last Updated: May 25, 2026

Neuro-rehabilitation Approach for Sudden Sensorineural Hearing Loss
09:44

Neuro-rehabilitation Approach for Sudden Sensorineural Hearing Loss

Published on: January 26, 2016

Systematic Hearing Performance Evaluation Process for Adolescents with Cochlear Implantation at Early Ages
06:04

Systematic Hearing Performance Evaluation Process for Adolescents with Cochlear Implantation at Early Ages

Published on: March 24, 2023

An Automated System for Sound Localization Testing in Hearing-Impaired Listeners
07:52

An Automated System for Sound Localization Testing in Hearing-Impaired Listeners

Published on: March 13, 2026

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Proofreading DNA polymerase fusions offer advantages like speed and fidelity over Taq-based enzymes for PCR.
  • Current limitations restrict their use to small to mid-range DNA targets.
  • Taq blends were previously the primary method for long-range PCR.

Purpose of the Study:

  • To present a modified protocol for amplifying long genomic targets (>20 kb) using proofreading DNA polymerase fusions.
  • To overcome limitations associated with traditional Taq blends for long-range PCR.
  • To enable efficient generation of high-molecular-weight amplicons.

Main Methods:

  • Utilizing a modified protocol with a proofreading DNA polymerase fusion.
  • Targeting genomic DNA fragments exceeding 20 kb.
  • Optimizing conditions for amplification of large DNA targets.

Main Results:

  • Successful amplification of genomic targets greater than 20 kb using the modified protocol.
  • Generation of high-molecular-weight amplicons within a single day.
  • Expectation of a significant proportion of error-free amplicons.

Conclusions:

  • The modified protocol effectively extends the utility of proofreading DNA polymerase fusions for long-range PCR.
  • This method provides a faster and potentially more accurate alternative to Taq blends for amplifying large genomic DNA fragments.
  • Enables efficient analysis of large amplicons in a single day.