[Concanavalin A binding sites on the cell membrane and other membrane structures of alveolar macrophages]

Concanavalin-A points of linkage were positively detected on cell membranes of alveolar and peritoneal macrophages by means of Con-A ferritin conjugate. Quantitative conclusions were drawn from these findings with regard to the number of mannose and glucose residues per 1 micron 2 of membrane area. With different incubation periods, 15, 25, and 45 minutes, various distribution patterns of ferritin molecules were recorded. They were diffusely distributed in cytoplasma as well as on the outer nuclear membrane. Ferritin particles were identified also on vacuolar membranes and in direct contact with lysosomes.