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Updated: Jun 7, 2026
GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
Published on: May 19, 2012
[Determination of ethyl glucuronide in blood and urine by LC-MS/MS]
Hui Zhao1, Xian-yi Zhuo, Hui Yan
1Shanghai Medical College, Fudan University, Shanghai 200032, China.
Objective:
To develop a method for determining ethyl glucuronide (EtG) in blood and urine by liquid chromatograph coupled with tandem mass spectrometer (LC-MS/MS).
Methods:
After blood and urine de-proteined by acetonitrile, the supernate obtained from a centrifuge was analyzed by LC-MS/MS.
Results:
Determination limit of EtG in both blood and urine was 0.05 pg/mL, with a linear range of 0.10-5.00 microg/mL (r > 0.999). Accuracy in both matrixes was 95%-109%. Inter- and intra-day RSD were less than 12%. The method showed an excellent performance when it was used to analyze authentic blood and urine samples for EtG.
Conclusion:
The method is capable for blood and urine EtG analysis.
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