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Updated: Jun 7, 2026
Attaching Biological Probes to Silica Optical Biosensors Using Silane Coupling Agents
Published on: May 1, 2012
Optimization of silica surface with nanosize holes for immobilization of biomolecules and analysis of their
Subash C B Gopinath1, Koichi Awazu, Makoto Fujimaki
1Photonics Research Institute, Bio-Photonics group, National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8562, Japan. gopi-subashchandrabose@aist.go.jp
Abstract:
An evanescent-field-coupled waveguide-mode sensor of the Kretschmann configuration with a silica waveguide having nanoscale holes is an ideal tool for detection of bimolecular reactions. In the present research, an optimized surface of the sensor with cylindrical nanoscale holes was modified with sodium (1-{[6-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)hexanoyl]oxy}-2,5-dioxopyrrolidine-3-sulfonate) (Sulfo-EMCS) to facilitate the attachment of biomolecules; the resulting surface could be cleaned for reuse simply by changing the pH of the buffering solution. The modification is expected to be useful for wide range of molecular detection.
