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Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
Proteomic analysis of bovine nucleolus
Amrutlal K Patel1, Doug Olson, Suresh K Tikoo
1Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.
Genomics, Proteomics & Bioinformatics
|October 26, 2010
Summary
This study identified 311 proteins in bovine nucleoli, revealing key roles in ribosomal biogenesis and cell cycle control. It also found 22 novel proteins not previously seen in human nucleoli proteomic analysis.
Area of Science:
- Cell Biology
- Proteomics
- Molecular Biology
Background:
- The nucleolus is a vital subnuclear structure involved in numerous eukaryotic cellular processes.
- Understanding the nucleolus's function requires detailed knowledge of its protein composition.
Purpose of the Study:
- To investigate the proteomic composition of bovine nucleoli.
- To elucidate the structural and functional roles of nucleoli in bovine cells.
Main Methods:
- Nucleoli were isolated from Madin Darby bovine kidney cells.
- Proteomic analysis was performed using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
- Proteins were fractionated using SDS-PAGE and strong cation exchange chromatography.
Main Results:
- A total of 311 proteins were identified in the bovine nucleoli.
- Twenty-two proteins were identified that had not been previously found in human nucleoli proteomic studies.
- Bioinformatic analysis indicated the presence of proteins involved in ribosomal biogenesis, cell cycle control, and various regulatory processes.
Conclusions:
- The proteomic profile of bovine nucleoli supports their diverse functional roles.
- This study expands the known proteome of nucleoli, particularly in a bovine context.
- The findings provide a foundation for further research into nucleolar function in mammalian cells.
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