Related Experiment Videos
Radioimmunoassay of corticotropin-releasing hormone
Y Rückert1, W Rohde, J Furkert
1Department of Internal Medicine, Humboldt University Medical School (Charité), Academy of Sciences, GDR, Berlin.
Experimental and Clinical Endocrinology
|November 1, 1990
Abstract:
A specific double antibody radioimmunoassay (RIA) for human-Corticotropin-Releasing Hormone (hCRF) using an antibody to synthetic hCRF was established. This antibody allowed a usable range of 10 pg to 5 ng of CRF per ml in the assay. Comparing the efficiency of various plasma extraction procedures, the extraction with ice-cold methanol was found to be the most simple and rapid method with an extraction efficiency of more than 80%. The reliability of the radioimmunoassay was shown under physiological and pathophysiological conditions.