Related Experiment Video
Updated: Jun 7, 2026
Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers
Published on: February 10, 2023
Phytic acid protects porcine intestinal epithelial cells from deoxynivalenol (DON) cytotoxicity
Graziela Drociunas Pacheco1, Caio Abércio da Silva, Philippe Pinton
1Universidade Estadual de Londrina, Centro de Ciências Agrárias, Campus Universitário, Londrina, CX Postal 6001, Brazil.
Abstract:
The purpose of this study was to evaluate the effects of phytic acid (IP(6)) as a possible inhibitor of cellular damage induced by toxic substances such as mycotoxins on a porcine intestinal epithelial cell line (IPEC-1). We first observed that a dose of 5 mM phytic acid decreases cell viability and transepithelial electrical resistance (TEER) of cell monolayer. We next investigate the effect of non-cytotoxic dose of phytic acid on the deoxinivalenol (DON) induced decreased TEER. We showed that treatment with 0.5 mM or 1.0 mM phytic acid restores the decrease in TEER caused by 25 μM DON. In conclusion this study demonstrates that phytic acid decreased the negative effects of deoxynivalenol on the membrane integrity of the IPEC-1 intestinal epithelial cell line.
