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Comet Assay as an Indirect Measure of Systemic Oxidative Stress

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Published on: May 22, 2015

Age associated oxidative damage in lymphocytes.

Nandeslu Gautam¹, Subhasis Das¹, Santanu Kar Mahapatra¹

¹Immunology and Microbiology Laboratory; Department of Human Physiology with Community Health; Vidyasagar University; Midnapore, West Bengal India.

Oxidative Medicine and Cellular Longevity

|October 26, 2010

Summary

This summary is machine-generated.

Aging increases oxidative stress and damage in lymphocytes, shifting the oxidant-antioxidant balance. This age-related oxidative damage in lymphocytes is crucial for understanding immunosenescence.

Keywords:

aging antioxidant enzymes lymphocyte oxidative stress

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Area of Science:

Immunology
Gerontology
Biochemistry

Background:

Lymphocytes are key to the acquired immune system and may influence immunosenescence.
Aging is associated with increased oxidative stress due to free radical production and reduced antioxidant defenses.

Purpose of the Study:

To assess lipid-protein damage and antioxidant status in lymphocytes of healthy individuals.
To correlate oxidative damage with the aging process in lymphocytes.

Main Methods:

Isolated lymphocytes from healthy individuals across various age groups (11-60 years).
Measured lipid damage via Malondialdehyde (MDA) and protein damage via protein carbonyls (PC).
Assessed antioxidant status using Superoxide Dismutase (SOD), Catalase (CAT), and glutathione-related enzymes.

Main Results:

Elevated MDA and PC levels were observed in older subjects compared to younger ones, indicating increased oxidative damage.
The balance between oxidant and antioxidant systems in lymphocytes shifts towards accelerated oxidative damage with aging.
Increased oxidative stress in lymphocytes appears significant in the aging process and immunosenescence.

Conclusions:

Oxidative damage in lymphocytes increases with age.
The aging process alters the oxidant-antioxidant balance within lymphocytes.
Lymphocyte oxidative stress is a key factor in immunosenescence.