Search research articles

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

Recombinant DNA

Recombinant DNA

Recombinant DNA

Recombinant DNA

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Brain over muscle: central mechanisms predominate in gait impairment among older adults with type 2 diabetes mellitus.

Frontiers in aging neuroscience·2026

Same author

Association between chrononutrition patterns and metabolic dysfunction-associated steatotic liver disease in adolescents: a population-based study.

Eating and weight disorders : EWD·2026

Same author

Gait variability as a dual-pathway marker of cognitive-motor dysfunction in older adults with type 2 diabetes mellitus.

Frontiers in endocrinology·2026

Same author

Determinants of Liver Steatosis Progression in Chinese Children: A Prospective Cohort Study.

Diabetes, metabolic syndrome and obesity : targets and therapy·2026

Same author

An <i>Agrobacterium tumefaciens EHA105</i>-Based GA<i>A</i>NTRY Recipient Strain Generates High-Quality Transgenic <i>Arabidopsis</i> and Potato.

Microorganisms·2026

Same author

Introducing Pattern Recognition Receptors in Potato Confers Enhanced Resistance to Ralstonia solanacearum but Not a Vector Borne Pathogen.

Plant biotechnology journal·2025

Same journal

Rhizome differentiation is associated with metabolic specialization and rhizosphere microbial assembly in Rheum officinale Baill.

Plant cell reports·2026

Same journal

Transcription factor MdMYB6L represses anthocyanin biosynthesis of apple skin at low-altitude regions.

Plant cell reports·2026

Same journal

The transcriptional dynamics of TIFY/JAZ and BBX families in jasmonate signaling reveal SlBBX17 as a positive regulator of tomato defense.

Plant cell reports·2026

Same journal

CRISPR/Cas9-mediated DFR disruption suggests coordinated changes in flavonoid flux and development in Petunia × hybrida.

Plant cell reports·2026

Same journal

Development and application of KASP molecular marker for accelerated breeding of low-acid Cerasus humilis cultivars.

Plant cell reports·2026

Same journal

Identification of the R2R3-MYB transcription factor family in goji and LbaR2R3-MYB3 mediated wax biosynthesis enhancing powdery mildew resistance in tobacco.

Plant cell reports·2026

See all related articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 7, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

Recombinase technology: applications and possibilities.

Yueju Wang¹, Yuan-Yeu Yau, Donna Perkins-Balding

¹Department of Natural Sciences, Northeastern State University, Broken Arrow, OK 74014, USA.

Plant Cell Reports

|October 26, 2010

Summary

This summary is machine-generated.

Site-specific recombination offers advanced plant genomic engineering, enabling precise transgene insertion and removal of unwanted DNA. This technology overcomes limitations of random insertion, improving efficiency and addressing consumer concerns about marker genes.

More Related Videos

Principles of Site-Specific Recombinase (SSR) Technology

Principles of Site-Specific Recombinase (SSR) Technology

Published on: May 29, 2008

Related Experiment Videos

Last Updated: Jun 7, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

Principles of Site-Specific Recombinase (SSR) Technology

Principles of Site-Specific Recombinase (SSR) Technology

Published on: May 29, 2008

Area of Science:

Plant biotechnology
Genomic engineering
Molecular biology

Background:

Traditional transgenesis involves random transgene insertion, leading to unpredictable expression and lengthy screening processes.
Antibiotic resistance genes used as markers in traditional methods raise environmental and consumer safety concerns.
Recombinase technology has evolved over three decades for genomic manipulation.

Purpose of the Study:

To review the diverse applications of site-specific recombination in plant genome engineering.
To present strategies for using multiple recombinase systems for precise transgene integration and marker gene removal.
To highlight the advantages of site-specific recombination over traditional methods.

Main Methods:

Review of existing literature on site-specific recombination in plants.
Discussion of recombinase applications in nuclear and plastid genomes.
Exploration of combined multiple recombinase systems for complex genome engineering tasks.

Main Results:

Site-specific recombination enables precise single-copy transgene insertion into predetermined genomic loci.
This technology effectively removes unwanted DNA, including selectable marker genes.
Recombinases can perform site-specific recombination in non-nuclear targets like the plastid genome.

Conclusions:

Site-specific recombination is a powerful tool for precise and efficient plant genomic engineering.
It offers solutions for predictable transgene expression and eliminates concerns associated with residual marker genes.
Advanced strategies using multiple recombinase systems can further refine transgene integration and removal processes.