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The effects of the preincubation with vitamin B2-acid on ferredoxin-NADP+ reductase
1Department of Chemistry, Faculty of Science and Engineering, Ritsumeikan University, Kyoto, Japan.
Journal of Nutritional Science and Vitaminology
|October 1, 1990
Abstract:
The effects of vitamin B2-acid on both cytochrome c reductase and diaphorase activities of ferredoxin-NADP+ reductase [EC 1.18.1.2] were investigated with enzyme kinetics. Vitamin B2-acid was shown to serve as an electron carrier for the two activities, as well as riboflavin or flavin mononucleotide (FMN). The two activities, however, were irreversibly affected by the preincubation of the enzyme with vitamin B2-acid under certain conditions, while riboflavin or FMN did not show such effects.