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Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

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Related Experiment Video

Updated: Jun 7, 2026

Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry

Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry

Published on: August 7, 2012

Salivary peptidomics.

Francisco Amado¹, Maria João Calheiros Lobo, Pedro Domingues

¹Department of Chemistry, University of Aveiro, 3810-193, Aveiro, Portugal. famado@ua.pt

Expert Review of Proteomics

|October 27, 2010

Summary

Saliva analysis reveals over 2000 peptides, the salivary peptidome, with many originating from non-glandular sources. Standardizing peptide nomenclature is crucial for clinical applications.

Area of Science:

Biochemistry
Proteomics
Clinical Diagnostics

Background:

Saliva contains a complex salivary peptidome (low molecular weight peptides), comprising 40-50% of total proteins.
Over 2000 peptides exist in saliva, with a significant portion originating from non-glandular sources like epithelial cells.
Proteolysis is a major source of salivary peptides, with proline-rich proteins (PRPs), statherin, and histatins being highly susceptible.

Purpose of the Study:

To harmonize the nomenclature of the salivary peptidome according to International Union of Pure and Applied Chemistry (IUPAC) recommendations.
To highlight the importance of specific collection, preservation, and analysis methods for salivary peptides.
To underscore the diagnostic potential of saliva for both oral and systemic diseases.

Main Methods:

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Last Updated: Jun 7, 2026

Sampling Human Indigenous Saliva Peptidome Using a Lollipop-Like Ultrafiltration Probe: Simplify and Enhance Peptide Detection for Clinical Mass Spectrometry

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Identification and characterization of salivary peptides.
Analysis of protein cleavage sites and involved proteases.
Review of existing literature on salivary peptidome composition and nomenclature.

Main Results:

The salivary peptidome exceeds 2000 identified peptides, with only 400-600 originating directly from salivary glands.
Specific cleavage sites (e.g., Gln-Gly in PRPs, Tyr-Gly in histatin 1) and proteases were identified.
Saliva offers a non-invasive alternative to blood or urine for diagnosing various oral and systemic conditions.

Conclusions:

Standardized nomenclature is essential for consistent research and clinical application of salivary peptidome data.
Saliva analysis provides valuable insights into oral health and holds potential for systemic disease diagnosis.
Precise methodologies are required for accurate salivary peptide analysis due to the fluid's complexity.