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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Natural glycan microarrays.

Emanuela Lonardi1, Crina I A Balog, André M Deelder

  • 1Biomolecular Mass Spectrometry Unit, Department of Parasitology, PO Box 9600, 2300 RC Leiden, The Netherlands.

Expert Review of Proteomics
|October 27, 2010
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Summary

Natural glycan microarrays offer powerful screening for protein-carbohydrate interactions, analyzing numerous glycans efficiently. This review compares strategies for isolating, characterizing, and applying these natural glycan arrays.

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Area of Science:

  • Glycobiology
  • Biochemistry
  • Analytical Chemistry

Background:

  • Glycan microarrays are advanced tools for studying protein-carbohydrate interactions.
  • They enable high-throughput analysis of hundreds to thousands of glycans simultaneously.
  • Natural glycan microarrays utilize glycans from natural sources, including known and novel structures.

Purpose of the Study:

  • To compare various strategies for developing and utilizing natural glycan microarrays.
  • To provide an overview of methods for glycan isolation, modification, and immobilization.
  • To discuss applications in studying carbohydrate-binding proteins like lectins and antibodies.

Main Methods:

  • Review of strategies for glycan isolation, derivatization, fractionation, and immobilization.
  • Overview of detection methods including fluorescence analysis and surface plasmon resonance.
  • Discussion of glycan density and multivalency effects on binding.

Main Results:

  • Natural glycan microarrays offer efficient screening of protein-carbohydrate interactions.
  • Diverse strategies exist for preparing and analyzing natural glycan probes.
  • Key factors like glycan density and multivalency influence binding outcomes.

Conclusions:

  • Natural glycan microarrays are valuable for dissecting complex carbohydrate-binding events.
  • The choice of microarray strategy depends on the specific research question and available resources.
  • These arrays facilitate the study of lectin and antibody specificities.