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Related Experiment Video

Updated: Jun 7, 2026

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture
07:44

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

Published on: March 3, 2023

Method for decellularizing skeletal muscle without detergents or proteolytic enzymes.

Allison R Gillies1, Lucas R Smith, Richard L Lieber

  • 1Department of Bioengineering, University of California San Diego, La Jolla, California 92093, USA.

Tissue Engineering. Part C, Methods
|October 27, 2010
PubMed
Summary

Researchers developed a new method to create decellularized skeletal muscle scaffolds. This technique preserves the extracellular matrix (ECM) composition and mechanical properties, offering a better model for studying muscle diseases.

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Last Updated: Jun 7, 2026

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture
07:44

Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture

Published on: March 3, 2023

Decellularization-Based Quantification of Skeletal Muscle Fatty Infiltration
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Published on: June 9, 2023

Enzymatic Isolation of Skeletal Muscle Interstitial Extracellular Vesicles
08:50

Enzymatic Isolation of Skeletal Muscle Interstitial Extracellular Vesicles

Published on: February 7, 2025

Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Muscle Physiology

Background:

  • Decellularized skeletal muscle ECM is valuable for studying muscle diseases.
  • Existing decellularization methods can degrade ECM components.
  • Need for methods preserving ECM structure and biochemistry.

Purpose of the Study:

  • Develop a novel decellularization method for skeletal muscle.
  • Preserve native ECM biochemical composition and structure.
  • Create a functional ECM scaffold for research.

Main Methods:

  • Sequential incubation of mouse tibialis anterior muscles.
  • Used latrunculin B, high ionic strength salt solution, and DNase I.
  • Avoided proteases and detergents to protect ECM.

Main Results:

  • Complete DNA removal confirmed by staining and quantification.
  • Biochemical analysis showed no collagen loss, slight GAG reduction.
  • Western blot confirmed actin/myosin removal; SEM showed myofiber removal.
  • Mechanical testing revealed nonlinear behavior similar to intact muscle.

Conclusions:

  • Novel protocol successfully decellularizes skeletal muscle.
  • Preserves ECM composition and mechanical function.
  • Provides a robust scaffold for studying muscle pathologies.