Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunoelectrophoretic studies on pig intestinal brush border proteins.

E M Danielsen, H Sjöström, O Norén

    Biochimica Et Biophysica Acta
    |October 26, 1977
    PubMed
    Summary

    This study developed antibodies against pig intestinal brush border proteins. These antibodies identified eight specific hydrolytic enzymes, aiding in understanding enterocyte function.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Colonic Health in Hospitalized Horses Treated with Non-Steroidal Anti-Inflammatory Drugs - A Preliminary Study.

    Journal of equine veterinary science·2021
    Same author

    Oral leukoplakia-to treat or not to treat.

    Oral diseases·2016
    Same author

    Molecular profiling of tumour budding implicates TGFβ-mediated epithelial-mesenchymal transition as a therapeutic target in oral squamous cell carcinoma.

    The Journal of pathology·2015
    Same author

    Life cycle assessment of energy self-sufficiency systems based on agricultural residues for organic arable farms.

    Bioresource technology·2010
    Same author

    TP53 mutations in clinically normal mucosa adjacent to oral carcinomas.

    Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·2010
    Same author

    Lipid raft organization and function in the small intestinal brush border.

    Journal of physiology and biochemistry·2009

    Area of Science:

    • Gastroenterology
    • Biochemistry
    • Immunology

    Background:

    • Brush borders are crucial for nutrient absorption in the small intestine.
    • Understanding the protein composition of brush borders is key to deciphering enterocyte function.
    • Specific enzymes within brush borders play vital roles in digestion.

    Purpose of the Study:

    • To generate specific antibodies against pig intestinal brush border membrane proteins.
    • To utilize these antibodies for identifying and characterizing brush border enzymes.
    • To contribute to the understanding of the molecular composition of the enterocyte brush border.

    Main Methods:

    • Preparation of brush borders from pig intestinal mucosa.
    • Solubilization of membrane proteins using Triton X-100 or papain.

    Related Experiment Videos

  • Antisera production in rabbits and isolation of immunoglobulin G (IgG) fractions.
  • Immunofluorescence staining to confirm antibody specificity for brush borders.
  • Immunoelectrophoretic analysis of brush border proteins.
  • Histochemical staining to identify enzymatic activities.

    • Main Results:

    • Successfully raised specific antibodies against pig intestinal brush border proteins.
    • Immunofluorescence confirmed antibody localization to the enterocyte brush border.
    • Immunoelectrophoresis identified eight known hydrolytic enzyme activities: microsomal aminopeptidase, aspartate aminopeptidase, dipeptidyl peptidase IV, lactase, glucoamylase, sucrase, isomaltase, and alkaline phosphatase.
    • At least four unidentified immunoprecipitates were also detected.

    Conclusions:

    • The generated antibodies are specific tools for studying pig intestinal brush border proteins.
    • The study successfully identified key hydrolytic enzymes present in the brush border.
    • Further investigation is needed to identify the unknown immunoprecipitated proteins.