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Related Concept Videos

Inflammation01:38

Inflammation

Overview
Atherosclerosis III: Management01:26

Atherosclerosis III: Management

Management of atherosclerosis involves an integrated strategy encompassing pharmacological treatment, surgical interventions, lifestyle changes, and nutrition therapy to address the multifactorial nature of the disease.Pharmacological TherapyA cornerstone of atherosclerosis management is the use of pharmacological agents. Statins, such as atorvastatin, are pivotal in inhibiting HMG-CoA reductase, an enzyme that catalyzes an initial step in cholesterol synthesis in the liver. This reduction in...
Atherosclerosis I: Introduction01:30

Atherosclerosis I: Introduction

Atherosclerosis is a progressive disorder characterized by the buildup of plaques on the arterial inner wall, causing them to narrow and harden over time. These plaques comprise lipids, calcium, blood components, carbohydrates, and fibrous tissue. The process primarily affects the intima of large and medium-sized arteries, reducing blood flow in any artery.Etiology and risk factorsThe cause of atherosclerosis is multifactorial, involving a complex interplay among endothelial injury, lipid...
Coronary Artery Disease II: Pathophysiology01:26

Coronary Artery Disease II: Pathophysiology

Coronary Artery Disease (CAD) originates from a series of events that impair the function of coronary arteries, the blood vessels responsible for delivering oxygen-rich blood to the heart muscle. The pathophysiology of CAD is closely linked to atherosclerosis, a chronic inflammatory and lipid-driven condition affecting the vascular endothelium.1. Endothelial DamageThe process begins with damage to the vascular endothelium, which serves as a protective barrier between the blood and the vessel...
Atherosclerosis II: Clinical Manifestations and Diagnostic Tests01:27

Atherosclerosis II: Clinical Manifestations and Diagnostic Tests

Atherosclerosis is a progressive disorder that leads to the thickening and narrowing of arterial walls due to plaque buildup. This condition can cause various symptoms depending on the arteries affected:Coronary Artery Disease (CAD): This condition affects the coronary arteries and may lead to chest pain (angina), shortness of breath (dyspnea), heart attacks, and other heart disease symptoms.Cerebrovascular Disease: This affects blood flow to the brain, causing transient ischemic attacks (TIAs)...
Coronary Artery Disease I: Introduction01:30

Coronary Artery Disease I: Introduction

Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...

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Related Experiment Video

Updated: Jun 7, 2026

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
07:46

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Published on: April 3, 2017

Selective macrophage ascorbate deficiency suppresses early atherosclerosis.

Vladimir R Babaev1, Richard R Whitesell, Liying Li

  • 1Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Free Radical Biology & Medicine
|October 27, 2010
PubMed
Summary

Severe ascorbic acid deficiency in macrophages surprisingly reduced atherosclerosis progression in mice. Macrophages lacking the ascorbate transporter (SVCT2) showed increased apoptosis and reduced inflammation, impacting early lesion development.

Related Experiment Videos

Last Updated: Jun 7, 2026

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
07:46

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation

Published on: April 3, 2017

Area of Science:

  • Biomedical Science
  • Cardiovascular Research
  • Cell Biology

Background:

  • Atherosclerosis is a chronic inflammatory disease.
  • Macrophages play a key role in atherosclerotic plaque development.
  • The role of severe ascorbic acid deficiency in macrophage function and atherosclerosis is not fully understood.

Purpose of the Study:

  • To investigate the impact of selective ascorbic acid deficiency in macrophages on the progression of early atherosclerosis.
  • To determine how lack of the ascorbate transporter (SVCT2) in macrophages affects their function and inflammatory responses.

Main Methods:

  • Generated apolipoprotein E-deficient (apoE(-/-)) mice lacking SVCT2 in hematopoietic cells via fetal liver cell transplantation.
  • Assessed aortic atherosclerosis, lesion macrophage content, and macrophage apoptosis after 13 weeks.
  • Analyzed peritoneal macrophage function, including response to H(2)O(2), gene expression, and signaling pathways (NF-κB, HIF-1α).

Main Results:

  • Mice with SVCT2-deficient macrophages exhibited significantly less aortic atherosclerosis and fewer lesion macrophages.
  • Macrophage apoptosis was increased in SVCT2-deficient mice.
  • Ascorbate-deficient macrophages showed impaired mitochondrial function, reduced inflammatory gene expression (COX-2, IL1β, IL6), and decreased NF-κB activation.

Conclusions:

  • Severe ascorbic acid deficiency in macrophages does not exacerbate, but rather attenuates, early atherosclerosis progression.
  • Macrophage SVCT2 deficiency leads to increased apoptosis and reduced inflammatory signaling, impacting lesion development.
  • These findings suggest a complex role for ascorbic acid in macrophage-mediated inflammatory processes relevant to cardiovascular disease.