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Updated: Jun 7, 2026
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The Use of a β-lactamase-based Conductimetric Biosensor Assay to Detect Biomolecular Interactions
Published on: February 1, 2018
Purification and biochemical characterization of IMP-13 metallo-beta-lactamase
Gisela Santella1, Jean-Denis Docquier, Gabriel Gutkind
1Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
Antimicrobial Agents and Chemotherapy
|October 27, 2010
Abstract:
The IMP-13 metallo-β-lactamase was overproduced in Escherichia coli BL21(DE3) and purified by chromatography. Analysis of kinetic parameters revealed some notable differences with other IMP-type enzymes, noteworthily a higher catalytic efficiency toward ticarcillin and piperacillin and a marked preference for imipenem over meropenem.
