Related Experiment Video
Updated: Jun 7, 2026

11:52
Single Particle Cryo-Electron Microscopy: From Sample to Structure
Published on: May 29, 2021
Elementary steps at the surface of ice crystals visualized by advanced optical microscopy
Gen Sazaki1, Salvador Zepeda, Shunichi Nakatsubo
1Institute of Low Temperature Science, Hokkaido University, N19-W8, Kita-ku, Sapporo 060-0819, Japan. sazaki@lowtem.hokudai.ac.jp
Summary
Researchers visualized ice crystal growth steps directly at air-ice interfaces using advanced microscopy. This breakthrough offers new insights into ice crystal surface dynamics and phase transitions.
Area of Science:
- Materials Science
- Surface Science
- Crystallography
Background:
- Understanding ice crystal surfaces is crucial for various natural phenomena.
- Molecular-level insights into ice growth are essential for numerous scientific fields.
Purpose of the Study:
- To directly visualize elementary steps and dynamic behavior of ice crystals at air-ice interfaces.
- To investigate the growth mechanisms of two-dimensional islands and spiral growth steps on ice surfaces.
Main Methods:
- Utilized laser confocal microscopy combined with differential interference contrast microscopy.
- Observed the appearance, lateral growth, and coalescence of two-dimensional islands on ice surfaces.
Main Results:
- Successfully visualized elementary steps of ice crystals with elementary heights of 0.37 nm (basal) and 0.39 nm (prism) faces.
- Observed spiral growth steps generated by screw dislocations on both basal and prism faces.
- Determined that the step ledge energy of a prism face is approximately 1/20 of that on a basal face.
Conclusions:
- The study provides direct optical evidence of elementary steps and two-dimensional island growth on ice surfaces.
- Findings support classical nucleation theory for elementary height islands and explain spiral growth step behavior.
- The observed differences in step ledge energy correlate with known roughening transition behaviors of ice faces.

