Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Telomeres and Telomerase

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.

Telomeres and Telomerase

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.

Replicative Cell Senescence

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Eukaryotes

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Addendum: Telomouse-a mouse model with human-length telomeres generated by a single amino acid change in RTEL1.

Nature communications·2026

Same author

Identification of ADAR1i-124: The first effective A-to-I RNA editing inhibitor with promising cancer therapeutic potential.

iScience·2026

Same author

Narrow-spectrum drug repurposing: targeting Gardnerella vaginalis biofilms associated with bacterial vaginosis.

Journal of applied microbiology·2026

Same author

Separation of telomere protection from length regulation by two different point mutations at amino acid 492 of RTEL1.

Nucleic acids research·2025

Same author

The Effects of Glucagon-Like Peptide-1 Agonist Therapy on Risk of Infection, Fracture, and Early Revision in Primary Total Joint Arthroplasty.

The Journal of the American Academy of Orthopaedic Surgeons·2025

Same author

Improving quality and outcomes of extracorporeal cardiopulmonary resuscitation in refractory cardiac arrest: the Phoenix ECPR project.

BMJ open quality·2025

Search research articles

Related Experiment Video

Updated: Jun 7, 2026

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

Insights into Cdc13 dependent telomere length regulation.

Mark Mason¹, Emmanuel Skordalakes

¹The Wistar Institute, Philadelphia, PA 19103, USA.

|October 27, 2010

Summary

Cdc13 protein caps chromosome ends in yeast, preventing aging and cancer. This telomere protection is crucial for cell viability and regulates cell division, offering potential therapeutic targets.

Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

Cdc13 is a single-stranded telomere binding protein essential for budding yeast viability.
It protects chromosome ends from fusion and degradation, preventing genomic instability and senescence.
Cdc13 regulates telomere length by controlling telomerase access to telomeric overhangs.

Purpose of the Study:

To elucidate the role of Cdc13 in maintaining telomere integrity and regulating cell division.
To explore the implications of Cdc13 function in aging and cancer prevention.
To identify Cdc13 and its homologs as potential therapeutic targets for age-related diseases and cancer.

Main Methods:

The study focuses on the functional characterization of Cdc13 in budding yeast.

More Related Videos

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Published on: April 21, 2023

Related Experiment Videos

Last Updated: Jun 7, 2026

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Optimization of Performance Parameters of the TAGGG Telomere Length Assay

Published on: April 21, 2023

Investigated Cdc13's role in telomere capping and its interaction with telomerase.

Comparative analysis with human homologs CTC1 and POT1.

Main Results:

Cdc13 is vital for capping chromosome ends, preventing end-to-end fusions and degradation.
It plays a dual role in telomere length regulation by modulating telomerase activity.
Dysfunctional capping can lead to genomic instability, senescence, or uncontrolled proliferation.
Human homologs CTC1 and POT1 share functional similarities with Cdc13.

Conclusions:

Cdc13 is a critical guardian of the genome, essential for preventing aging and cancer.
Its function in telomere maintenance highlights its significance in cell viability and longevity.
Cdc13 and its homologs represent promising targets for developing novel cancer and age-related therapies.