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Related Experiment Video

Updated: Jun 7, 2026

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants
09:20

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus (AAV) Capsid Variants

Published on: October 18, 2022

Establishment of an AAV reverse infection-based array.

Xiaoyan Dong1, Wenhong Tian, Gang Wang

  • 1State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai, China.

Plos One
|October 27, 2010
PubMed
Summary
This summary is machine-generated.

A new Adeno-associated virus (AAV) reverse infection method enables high-throughput gene transduction on 96-well plates. This platform facilitates efficient screening and biological assays by optimizing AAV vector delivery and cell response.

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Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling

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Engineering and Evolution of Synthetic Adeno-Associated Virus (AAV) Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

Area of Science:

  • Molecular Biology
  • Gene Therapy
  • Cell Biology

Background:

  • High-throughput gene transduction is essential for biological screening.
  • Adeno-associated virus (AAV) vectors are widely used but limited in in vitro high-throughput applications.

Purpose of the Study:

  • To develop a convenient and efficient method for high-throughput gene transduction using AAV vectors.
  • To establish an Adeno-associated virus (AAV) reverse infection (RI) array for cell biological assays.

Main Methods:

  • Developed an AAV reverse infection (RI) method using quantified recombinant AAVs (rAAVs) pre-coated onto 96-well plates.
  • Evaluated pre-coated rAAV particle numbers, cell loading, and temperature stability.
  • Compared transduction efficiencies of six rAAV serotypes/hybrids across multiple cell lines.

Main Results:

  • Adeno-associated virus (AAV) serotypes 1 and 2 demonstrated high transduction efficiency, suitable for the RI array.
  • Sodium butyrate treatment significantly enhanced reporter gene expression, indicating system responsiveness.
  • The method successfully reflected biological responses to treatments.

Conclusions:

  • A novel, highly efficient gene transduction array using AAV reverse infection was established.
  • This method can be developed into a versatile platform for cell biological assays.
  • The system offers a valuable tool for biological screening and gene therapy research.