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Related Experiment Video

Updated: Jun 7, 2026

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
12:53

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Published on: May 19, 2018

Nodeomics: pathogen detection in vertebrate lymph nodes using meta-transcriptomics.

Nicola E Wittekindt1, Abinash Padhi, Stephan C Schuster

  • 1Department of Biochemistry and Molecular Biology, Center for Comparative Genomics and Bioinformatics, The Pennsylvania State University, University Park, Pennsylvania, United States of America.

Plos One
|October 27, 2010
PubMed
Summary
This summary is machine-generated.

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Researchers explored the microbial communities in mule deer lymph nodes using meta-transcriptomics. This study identified novel bacteria and viruses, advancing wildlife microbiome research and pathogen detection.

Area of Science:

  • Microbiology
  • Wildlife Health
  • Genomics

Background:

  • Emerging infectious diseases from wildlife necessitate understanding host-associated microbial communities.
  • Traditional diagnostics are insufficient for comprehensive wildlife microbiome analysis.
  • Mule deer (Odocoileus hemionus) serve as a model for investigating wildlife microbial biota.

Purpose of the Study:

  • To characterize the microbial biota in healthy mule deer lymph nodes.
  • To evaluate the efficacy of meta-transcriptomics for wildlife microbiome studies.
  • To identify novel microbial species with potential pathogenic significance.

Main Methods:

  • Meta-transcriptomic analysis of retropharyngeal lymph node RNA from mule deer.
  • Sequencing using Roche-454 Life Sciences technology.

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High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

Related Experiment Videos

Last Updated: Jun 7, 2026

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species
12:53

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Published on: May 19, 2018

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
14:58

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

  • Taxonomic profiling of protein-coding and 16S ribosomal RNA (rRNA) sequences against specialized databases.
  • Amplicon sequencing of the V6 region of 16S rRNA for comparative diversity assessment.

    • Main Results:

    • Detection of representatives from all bacterial phyla, indicating viable lymph node microbiota.
    • Identification of two novel proteobacterial species: a Helicobacter and an Acinetobacter.
    • Discovery of a novel gamma retrovirus and other viral families (Poxviridae, Retroviridae).
    • Meta-transcriptomics revealed higher overall bacterial taxonomic diversity compared to 16S rRNA amplicon sequencing.

    Conclusions:

    • Meta-transcriptomics provides a comprehensive view of wildlife host-associated microbial diversity.
    • This approach facilitates the discovery of novel microorganisms in wildlife tissues.
    • The findings enhance the potential for detecting organisms with zoonotic or animal health implications.