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Updated: Jun 7, 2026

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses

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Host genetics and HIV-1: the final phase?

Jacques Fellay¹, Kevin V Shianna, Amalio Telenti

¹Center for Human Genome Variation, Duke University School of Medicine, Durham, North Carolina, United States of America. jacques.fellay@duke.edu

|October 27, 2010

Summary

Human genetics research is advancing, particularly in understanding HIV host genetics. Genome-wide studies and new approaches are needed to explain individual differences in HIV-1 infection response.

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Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

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Published on: October 16, 2018

Area of Science:

Human genetics
Immunology
Virology

Background:

Candidate gene analyses for HIV host genetics yielded equivocal results.
Recent genome-wide association studies (GWAS) investigated HIV-1 plasma viral load and disease progression.
Large-scale studies (siRNA screens, transcriptomics, proteomics, comparative genomics) offer new insights into retroviral pathogenesis.

Purpose of the Study:

To highlight the critical transition in human genetics, especially HIV host genetics.
To emphasize the need for advanced genomic and systems biology approaches.
To address the unexplained inter-individual variability in HIV-1 infection response.

Main Methods:

Genome-wide association studies (GWAS)
siRNA screens
Transcriptome and proteome analysis
Comparative genomics

Main Results:

GWAS have begun to clarify genetic associations with HIV-1 viral load and disease progression.
Large-scale approaches are illuminating retroviral pathogenesis.
Significant inter-individual variability in HIV-1 response remains unexplained.

Conclusions:

Current understanding of HIV-1 host genetics is incomplete.
Advanced genome resequencing and systems biology are essential for future progress.
Further research is required to fully understand complex HIV-1-host interactions.