Related Experiment Video
Updated: Jun 7, 2026
Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
Published on: January 19, 2019
Coating electrospun chitosan nanofibers with polyelectrolyte multilayers using the polysaccharides heparin and
Jorge Almodóvar1, Matt J Kipper
1Department of Chemical and Biological Engineering, 1370 Campus Delivery, Colorado State University, Fort Collins, Colorado 80523-1370, USA.
Abstract:
A new method is presented for functionalizing electrospun nanofibers with GAGs and growth factors by PEM deposition. Electrospun chitosan nanofibers, spun from trifluoroacetic acid and dichloromethane, were coated with PEMs, using the polysaccharides heparin and N,N,N-trimethyl chitosan. FGF-2 was adsorbed on the PEM-coated nanofibers. Nanofiber neutralization, PEM construction, and FGF-2 adsorption were monitored using FT-IR spectroscopy and X-ray photoelectron spectroscopy. Alcian blue staining was used to confirm the presence of heparin. SEM was used to study nanofiber morphology.
