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Updated: Jun 7, 2026
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Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System
Published on: December 7, 2012
Impact of pathogen reduction technology and storage in platelet additive solutions on platelet function
Ana M Galan1, Miguel Lozano, Patricia Molina
1Servicio de Hemoterapia y Hemostasia, Hospital Clínic, C/ Villarroel 170, Barcelona 08036, Spain. agalan@clinic.ub.es
Transfusion
|October 28, 2010
Summary
Pathogen reduction technology (PRT) effectively preserves platelet concentrate (PC) properties in additive solutions for 5 days. PASIIIM better maintains platelet function than PASIII for 7-day storage.
Area of Science:
- Hematology
- Transfusion Medicine
- Biotechnology
Background:
- Limited research exists on pathogen reduction technology (PRT) effects on platelet (PLT) products stored in platelet additive solutions (PASs).
- This study evaluates Mirasol PRT and storage in PAS (PAS-III or PAS-IIIM) on platelet concentrate (PC) characteristics.
Purpose of the Study:
- To assess the impact of Mirasol PRT on analytical and functional parameters of platelet concentrates stored in PAS.
- To compare the efficacy of PAS-III and PAS-IIIM in preserving PRT-treated platelet concentrates.
Main Methods:
- Apheresis-derived PCs were divided into untreated (plasma or PAS storage) and PRT-treated (PAS storage) groups.
- Evaluated cell quality (pH, swirl, lactate, glucose), flow cytometry, and perfusion studies over 7 days.
- Assessed adhesive and aggregating functions using a perfusion system.
Main Results:
- Cell quality variables declined during storage; swirl was better maintained in plasma.
- PRT-treated PCs showed moderate glycoprotein Ib reduction by Day 7.
- PRT-treated PCs in PAS demonstrated comparable subendothelial reactivity to untreated PCs at Day 5.
- PASIIIM better preserved PRT-PC cohesive properties at Day 7 compared to PASIII.
Conclusions:
- Platelet properties are maintained in PRT-treated concentrates stored in PAS for up to 5 days.
- PASIIIM offers superior preservation of adhesive and cohesive functions for PRT-PCs over 7 days compared to PASIII.
- Further clinical studies are warranted to validate these in vitro findings.
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