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Updated: Jun 7, 2026
09:30
Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
Published on: September 13, 2018
Coevolution within a transcriptional network by compensatory trans and cis mutations
Dwight Kuo1, Katherine Licon, Sourav Bandyopadhyay
1Department of Bioengineering, University of California, San Diego, La Jolla, California 92093, USA.
Genome Research
|October 28, 2010
Summary
Compensatory mutations in yeast
Area of Science:
- Evolutionary biology
- Molecular biology
- Genetics
Background:
- Transcriptional networks evolve rapidly, with cis-acting DNA sequence variations often driving divergence.
- The interplay between cis-acting and trans-acting genetic changes in this process remains unclear.
Purpose of the Study:
- To investigate compensatory mutations within the yeast AP-1 transcriptional network.
- To understand how conserved transcriptional regulation is maintained despite genetic changes.
Main Methods:
- Comparative genomics of yeast species.
- Chromatin immunoprecipitation to analyze protein-DNA interactions.
- Analysis of cis-regulatory motifs and trans-acting factors.
Main Results:
- Identified recent trans mutation in Candida glabrata's AP-1 factor.
- Observed conserved gene regulation by AP-1 orthologs despite sequence differences.
- Discovered concurrent cis-regulatory motif changes compensating for trans mutations.
Conclusions:
- Demonstrated coevolution between a transcription factor and its cis-regulatory sites.
- Showcased a mechanism for maintaining transcriptional network stability through compensatory mutations.
- Provided insights into the rapid evolution of gene regulatory systems.
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