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Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Updated: Jun 7, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

A printed nanolitre-scale bacterial sensor array.

Sahar Melamed¹, Laura Ceriotti, Wilfried Weigel

¹Department of Plant and Environmental Sciences, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.

|October 28, 2010

Summary

This summary is machine-generated.

Researchers developed a novel method for printing whole-cell bacterial biosensor arrays. This technique immobilizes bacteria, maintaining their viability and sensing function for extended periods, enabling high-throughput applications.

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Area of Science:

Biotechnology
Microbial Engineering
Biosensor Technology

Background:

Growing interest in whole-cell biosensors and array technologies.
Need for integrated whole-cell array biosensors for diverse applications.

Purpose of the Study:

To demonstrate a method for creating whole-cell bacterial bioreporter arrays.
To investigate the immobilization, viability, and sensing activity of printed bacteria.

Main Methods:

Utilized a non-contact robotic printer for nanoliter-scale bacterial spot patterning.
Exploited bacterial adhesion to positively charged surfaces for immobilization.
Optimized immobilization through genetic modification (curli overproduction), media manipulation (osmotic stress, osmoprotectants), and surface chemistry (self-assembled monolayers).

Main Results:

Successfully immobilized and patterned Escherichia coli-based sensor bacteria.
Printed bacteria retained viability and biosensing activity for over 2 months at 4 °C.
Immobilization efficiency was significantly improved by optimizing multiple factors.

Conclusions:

Presented a viable methodology for manufacturing whole-cell sensor arrays.
The developed technique is suitable for diverse high-throughput applications.
This approach combines whole-cell biosensing with advanced array fabrication.