Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
Ion-Exchange Chromatography01:09

Ion-Exchange Chromatography

Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
Affinity and Avidity01:41

Affinity and Avidity

Overview
Types Of Column Chromatography01:29

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...
Immunocytochemistry and Immunohistochemistry01:22

Immunocytochemistry and Immunohistochemistry

Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Single Cells to Silicon: Emerging Technologies Transforming Monoclonal Antibody Discovery.

Antibodies (Basel, Switzerland)ยท2026
Same author

From lab to field: Transforming immunoassays for rapid microcystin-LR monitoring in Lough Neagh.

Ecotoxicology and environmental safetyยท2026
Same author

Updates to Guidance on Using Biacore for Protein-Protein Interactions Analysis.

Methods in molecular biology (Clifton, N.J.)ยท2026
Same author

Real-world impact of nirsevimab immunisation and maternal RSV vaccination against respiratory disease on emergency department attendances and admissions: a multinational retrospective analysis.

The Lancet regional health. Europeยท2026
Same author

Real-world impact of nirsevimab immunisation against respiratory disease on emergency department attendances and admissions among infants: a multinational retrospective analysis.

The Lancet regional health. Europeยท2025
Same author

A deep learning approach to case prioritisation of colorectal biopsies.

Histopathologyยท2024

Related Experiment Video

Updated: Jun 7, 2026

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
07:53

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

Immunoaffinity chromatography.

Jenny Fitzgerald1, Paul Leonard, Elaine Darcy

  • 1School of Biotechnology, Dublin City University, Dublin, Ireland.

Methods in Molecular Biology (Clifton, N.J.)
|October 28, 2010
PubMed
Summary
This summary is machine-generated.

Immunoaffinity chromatography (IAC) uses antibody-ligand binding for efficient purification. Optimizing parameters like affinity pairs and elution is key for successful antibody separation and diverse applications.

More Related Videos

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System
07:19

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

Published on: April 26, 2024

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
10:50

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Published on: March 9, 2010

Related Experiment Videos

Last Updated: Jun 7, 2026

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
07:53

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

Published on: August 16, 2019

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System
07:19

Affinity Purification of a 6X-His-Tagged Protein using a Fast Protein Liquid Chromatography System

Published on: April 26, 2024

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography
10:50

Isolation of Labile Multi-protein Complexes by in vivo Controlled Cellular Cross-Linking and Immuno-magnetic Affinity Chromatography

Published on: March 9, 2010

Area of Science:

  • Biochemistry
  • Separation Science
  • Immunotechnology

Background:

  • Antibody-based separation methods, including immunoaffinity chromatography (IAC), are vital for purifying biomolecules.
  • These techniques leverage specific antibody-ligand interactions for high efficiency.

Purpose of the Study:

  • To review the critical parameters for designing effective immunoaffinity chromatography (IAC) platforms.
  • To discuss antibody generation and applications in IAC.

Main Methods:

  • The review focuses on optimizing key experimental parameters for IAC.
  • Discussion includes biological affinity pairs, matrix supports, immobilization chemistry, and elution conditions.

Main Results:

  • Successful IAC design relies on optimizing the interplay between antibody-ligand affinity, matrix, coupling, and elution.
  • Optimized IAC enables efficient antibody isolation for various applications.

Conclusions:

  • Immunoaffinity chromatography is a powerful technique for antibody purification and isolation.
  • Careful optimization of experimental parameters is essential for maximizing IAC efficiency and applicability.