Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Systematic bias in point-of-care INR testing: comparative analysis of Abbott and Roche devices.

Clinical chemistry and laboratory medicine·2026
Same author

Accuracy of Factor VIII Assays for Measuring Damoctocog Alfa Pegol: A Comparative Analysis.

Haemophilia : the official journal of the World Federation of Hemophilia·2026
Same author

Reporting of D-dimer testing in venous thromboembolism diagnostic management studies: a scoping review.

Journal of thrombosis and haemostasis : JTH·2026
Same author

Measurement uncertainty of ISO 17511:2020 compliant and globally standardized PT/INR test results.

Research and practice in thrombosis and haemostasis·2026
Same author

Performance of Factor VIII Extended Half-Life Product Measurements in External Quality Assessment Programmes.

International journal of laboratory hematology·2026
Same author

Assay of Efanesoctocog Alfa in 200 Centres: Data From Collaborative NEQAS BC (United Kingdom) and ECAT (Netherlands) Exercise Autumn 2024.

Haemophilia : the official journal of the World Federation of Hemophilia·2025

Related Experiment Video

Updated: Jun 7, 2026

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
08:01

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

External quality assessment for thrombin generation tests: an exploration.

Cornelis Kluft1, Piet Meijer

  • 1ECAT Foundation, Leiden, The Netherlands. kluft@euronet.nl

Seminars in Thrombosis and Hemostasis
|October 28, 2010
PubMed
Summary

External Quality Control of Diagnostic Assays and Tests surveys reveal significant variability in thrombin generation tests (TGTs). Differences in microparticle levels and Factor XII activity impact TGT results, affecting clinical applicability.

More Related Videos

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis
08:50

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis

Published on: January 9, 2026

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization
06:28

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization

Published on: June 4, 2020

Related Experiment Videos

Last Updated: Jun 7, 2026

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well
08:01

The Nijmegen Hemostasis Assay: Simultaneous Fluorogenic Measurement of Thrombin and Plasmin Generation in a Single Well

Published on: February 27, 2026

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis
08:50

Thrombus Profiling Assay: A Microfluidics-Based Platform for Comprehensively Characterizing Biomechanical Thrombogenesis

Published on: January 9, 2026

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization
06:28

Leveraging Turbidity and Thromboelastography for Complementary Clot Characterization

Published on: June 4, 2020

Area of Science:

  • Clinical Chemistry
  • Hematology
  • Diagnostic Assay Development

Background:

  • External Quality Control of Diagnostic Assays and Tests (ECAT) surveys highlight substantial inter-laboratory variability in thrombin generation tests (TGTs).
  • Observed differences in TGT results, particularly time to peak (TTP) and area under the curve (AUC), exceed 30-fold across participating laboratories.
  • This variability necessitates understanding the impact of pre-analytical factors on TGT performance.

Purpose of the Study:

  • To investigate the influence of microparticles (MPs) and Factor XII (FXII) activity on TGT results.
  • To assess the interlaboratory variability of TGTs using different plasma matrices.
  • To correlate TGT performance with specific pre-analytical variables for improved diagnostic accuracy.

Main Methods:

  • Analysis of pooled normal plasmas, MP-depleted plasmas, and FXII-deficient patient plasma across 4-11 participating laboratories.
  • Measurement of time to peak (TTP) and area under the curve (AUC) as key TGT variables.
  • Evaluation of MP depletion effects and FXII deficiency impact on TTP and AUC, including comparison with contact activation inhibitors.

Main Results:

  • MP depletion increased TTP by up to 29% and decreased AUC by up to 38%, with significant individual laboratory differences.
  • FXII deficiency increased TTP in slow TGTs (248% to 331%) and gradually decreased AUC (up to 85%).
  • Interlaboratory variability ranged from 11% to 57%, primarily linked to the normal pooled plasma used.

Conclusions:

  • TGTs exhibit differential sensitivity to MPs and contact activation, impacting their clinical relevance.
  • Variability in TGTs is influenced by plasma composition, particularly MPs and FXII.
  • Future ECAT surveys should incorporate varied MP and contact activation samples to better characterize TGT variants and their clinical associations.