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[Production and characterization of a high affinity monoclonal antibody with digoxin and digitoxin specificity]
H U Simon1, W Hubl, W D Müller
1Institut für Klinische Immunologie, Friedrich-Schiller-Universität Jena.
Allergie Und Immunologie
|January 1, 1990
Abstract:
A monoclonal antibody with a high affinity for digoxin (KA = 5.5 x 10(10) M-1) and digitoxin KA = 5.0 x 10(10) M-1) was produced by somatic cell fusion. This antibody, designated 2A3, displayed little cross reactivity with other glucosides and no cross reactivity with endogenous steroids. It was shown that 2A3 is a suitable tool in an enzyme immunoassay for digoxin and digitoxin.