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Updated: Jun 7, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Published on: December 11, 2021
Interaction of CO dehydrogenase with the cytoplasmic membrane monitored by fluorescence correlation spectroscopy
Florian Spreitler1, Christian Brock, Astrid Pelzmann
1Experimentalphysik IV and Forschungszentrum für Bio-Makromoleküle, Universität Bayreuth, Universitätsstrasse 30, 95447 Bayreuth, Germany.
Abstract:
We have investigated the interaction of carbon monoxide dehydrogenase (CODH), an enzyme that catalyses the oxidation of CO in the aerobic eubacterium Oligotropha carboxidovorans, with the cytoplasmic membrane by using fluorescence correlation spectroscopy (FCS). Our results reveal that in vitro this interaction of CODH is specific for cytoplasmic membranes from CO-grown bacteria.

