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Structural studies on allergen RC-13 from Ricinus communis L.: isolation and characterization of a major glycopeptide
J G Silva Júnior1, M A Garcia, O L Machado
1Departamento de Bioquímica, Universidade Federal do Rio de Janeiro.
Anais Da Academia Brasileira De Ciencias
|March 1, 1990
Abstract:
A glycoprotein, RC-13, isolated from Ricinus communis seeds was reduced, S-alkylated and cleaved by trypsin. The tryptic digest was fractionated by ion-exchange chromatography and a glycopeptide was isolated and purified by high-voltage paper electrophoresis. When submitted to amino acid and carbohydrate analyses this major glycopeptide showed the following chemical composition: Lys1, Asp1, Thr2, Ser4, Glu1, Pro2, Gly2, Ala2, Val2, GlcN6, Man6 and Gal8. Hydrazynolysis positioned Ser as the C-terminal residue. It is postulated that this glycopeptide belongs to the C-terminal region of the allergen.