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[Study on the activity against influenza A virus with traditional Chinese medicine Kanggan granules]
Feng-huan Liu1, Ming-yuan Li, Jun Luo
1Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Objective:
To study the anti-influenza A virus effects of traditional Chinese medicine Kanggan granules in chicken embryo and BALB/c mice.
Methods:
The influenza A virus (H(1)N(1), FM1) was used in the experiments. FM1 was cultured in chicken embryo and the anti-FM1 activity of Kanggan granules was evaluated through the post-medication hemagglutination titer of FM1. In animal test, 120 healthy BALB/c mice were randomly divided into six groups, normal control, virus control, ribavirin, high-dosage, middle-dosage and low-dosage. The FM1 infection model was established by dripping FM1 into nasal cavity and then the appropriate treatments were prescribed. The effective anti- FM1 indices of Kanggan granules included survival status, protective percentage of death and life elongation percentage of mice infected with FM1.
Results:
The high and middle doses of Kanggan granules could inhibit the replication of FM1 remarkably in chicken embryo, and could reduced hemagglutination titer to 5 and 3 times. In animal experiments, all mice treated with Kanggan granules could improve the general status of infected mice, the protective percentages of death were 35.0% to 55.0%, the life elongation percentages were 73.0% to 88.9% and the minimal effective dose was 3.00 g/kg.
Conclusion:
Kanggan granules can inhibit the replication of influenza A virus and protect the mice infected with FM1.
Insights
Traditional Chinese medicine Kanggan granules demonstrate significant anti-influenza A virus activity. Studies show Kanggan granules inhibit viral replication and protect mice from influenza A virus infection.
Area of Science:
- Virology
- Immunology
- Pharmacology
Context:
- Influenza A virus (IAV) poses a significant threat to public health.
- Traditional Chinese Medicine (TCM) offers potential therapeutic avenues for viral infections.
- Kanggan granules are a TCM formulation with suspected antiviral properties.
Purpose:
- To investigate the efficacy of Kanggan granules against Influenza A virus (H1N1, FM1).
- To evaluate the antiviral effects in both in vitro (chicken embryo) and in vivo (BALB/c mice) models.
Summary:
- Kanggan granules significantly inhibited FM1 replication in chicken embryos, reducing hemagglutination titers.
- In mice, Kanggan granules improved general condition, with protective effects against mortality ranging from 35.0% to 55.0%.
- The minimal effective dose in mice was determined to be 3.00 g/kg, with life elongation percentages between 73.0% and 88.9%.
Impact:
- Provides scientific evidence for the anti-influenza A virus properties of Kanggan granules.
- Suggests Kanggan granules as a potential therapeutic agent for influenza A virus infections.
- Highlights the value of TCM in developing novel antiviral strategies.