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Updated: Jun 7, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
Published on: April 16, 2015
Thymocytes are activated by toluene inhalation through the transcription factors NF-κB, STAT5 and NF-AT
Jiqin Liu1, Yasuhiro Yoshida, Naoki Kunugita
1Department of Immunology, School of Medicine, University of Occupational and Environmental Health Japan, Kitakyushu 807-8555, Japan.
Abstract:
Toluene has been extensively examined for effects on the central nervous system. To investigate the influence of low-level inhalation of toluene on the naive immune cells, male C3H/HeN mice were exposed to filtered air (control) and 50 ppm of toluene for 3 weeks. Low-level exposure resulted in (1) increased proliferation of thymocytes, (2) IL-2 production induced in thymocytes and (3) activation of the transcription factors NF-κB, STAT5 and NF-AT in thymocytes. These results suggest that thymocytes are sensitive cells and T cell activators are candidates for biomarkers for low-level exposure to toluene on naive immune cells.
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