[On the standardization of new chemical risk evaluation with eye irritation test]

Three new chemicals, N-acyl glutamic acid as a stroma of cosmetics, an eye drops for myopia and 2, 4-dichloro-6-nitrophenylaminate (DCNP) as a new herbicide were evaluated with the eye irritation test. Japanese young rabbits were used, the right eye for test, the left as control. Before and after the test, the eye were stained with 2% sodium fluorescein and examined under slit-lamp microscope to observe the damage degree of cornea, and to record the reaction degree of conjunctiva and iris at the same time. Using the draize method to compare with three kinds of granding (method of bayard and hehir, of EPA and of NRC), the results were essentially the same. The severity of reaction to the eye irritation test is closely related to the kind of testing material and the concentration used and the length of time exposed. The standardization of eye irritation test is also discussed.