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Removal of sperm-coat from human spermatozoa by interaction with cervical mucus or a capacitating medium
M Rosselli1, M Marchini, G Soldati
1Andrology Laboratory, La Carita Hospital, Locarno, Switzerland.
Andrologia
|November 1, 1990
Abstract:
Human spermatozoa were labelled with cationized ferritin and their interaction with cervical mucus or a capacitating medium enriched with 3% bovine serum albumin (BSA) was investigated by transmission electron microscopy. It was found that the sperm-coat was removed from the surface of spermatozoa after incubation in the BSA-enriched capacitating medium, as well as after crossing a column filled with cervical mucus. These results suggest that both media have a quantitatively similar action in removing the sperm-coat.